Journal: Cell reports

Article Title: Motion Streak Neurons in the Mouse Visual Cortex

doi: 10.1016/j.celrep.2020.108617

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV1-Syn-GCaMP6f viral vector addgene 100837 Chemicals, Peptides, and Recombinant Proteins Carprofen Zoetis 141–199 Chlorprothixene hydrochloride Sigma C1671 Vetbond 3M 70200742529 Metabond Parkell S380 Isoflurane Covetrus 1169567761 Experimental Models: Organisms/Strains C57BL/6 bread at lead contact lab N/A Software and Algorithms Psychophysics Toolbox Psychtoolbox http://psychtoolbox.org/docs/Psychtoolbox MATLAB 2018a MathWorks https://www.mathworks.com/products/matlab.html LabVIEW 2017 National Instruments https://www.ni.com/en-us/shop/labview.html Other Microinjector Drummond Scientific Nanoject II CCD camera Bitran BU-61M 470 nm LED Prizmatix UHP-T-LA LCD monitor ViewSonic VA951S LCD monito MSI Optix G24C Temperature control FHC N/A Head Holding Device Narishige SR-AM Anesthesia Systems Kent scientific VetFlo Open in a separate window KEY RESOURCES TABLE.

Techniques: Plasmid Preparation, Recombinant, Software

Journal: Cell reports

Article Title: Ki67 is a Graded Rather than a Binary Marker of Proliferation versus Quiescence

doi: 10.1016/j.celrep.2018.06.110

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cas9 Nuclease V3 IDT Cat# 1081058 DMEM/F12 Thermo Fisher Cat# 11330–032 DMEM/F12 Phenol red free Thermo Fisher Cat# 11330–021 horse serum Thermo Fisher Cat# 16050–122 cholera toxin Sigma Cat# C8052–2mg epidermal growth factor Peprotech Cat# AF-100–15 Insulin Sigma Cat# I1882–200mg Hydrocortisone Sigma Cat# H0888–1g Critical Commercial Assays Click-iT EdU Alexa Fluor 647 Imaging Kit Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"C10340","term_id":"1535411","term_text":"C10340"}} C10340 Experimental Models: Cell Lines MCF-10A cell line ATCC Cat# CRL-10317, RRID:CVCL_0598 MCF10A H2B-mTurquoise DHB-mVenus Laboratory of Tobias Meyer ( Spencer et al., 2013 ) MCF10A mCitrine-Ki67 This study N/A MCF10A mCitrine-Ki67 H2B-mTurquoise DHB-mCherry This study N/A MCF10A mCitrine-Ki67 H2B-mTurquoise Geminin-mCherry This study N/A MCF7 Ki67-YFP p21-mKate Laboratory of Galit Lahav ( Stewart-Ornstein and Lahav, 2016 ) MCF7 Ki67-YFP p21-mKate H2B-mIFP DHB-mTurquoise2 This study N/A OVCAR-3 cell line ATCC Cat# HTB-161, RRID:CVCL_0465 A375 cell line ATCC Cat# CRL-1619, RRID:CVCL_0132 Oligonucleotides FZR1 DsiRNA IDT Cat# hs.Ri.FZR1.13.2 MYBL2 DsiRNA IDT Cat# hs.Ri.MYBL2.13.2 FOXM1 DsiRNA IDT Cat# hs.Ri.FOXM1.13.1 Negative Control DsiRNA IDT Cat# 51–01-14–04 MKI67 crRNA (AAUGUGGCCCACGAG) IDT N/A CRISPR-Cas9 tracrRNA IDT Cat# 1072532 REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms MATLAB Mathworks SCR_001622 Other 96 well plate Greiner bio-one Cat# 655090 96 well glass bottom plate Cellvis Cat# P96–1.5H-N Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Imaging, Negative Control, CRISPR, Software